Proteolytic Enzymes. V. Further Application of p-Nitrophenyl p-Amidinobenzoate as a Titrant of Trypsin
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منابع مشابه
Benzyl p-guanidinothiobenzoate hydrochloride, a new active-site titrant for trypsin and trypsin-like enzymes.
Benzyl p-guanidinothiobenzoate hydrochloride was synthesized and demonstrated to be useful for active-site titration of bovine trypsin, bovine thrombin, human lung tryptase, bovine activated protein C, human Factor XIIa fragment and bovine Factor Xa beta. The titration is based on rapid formation of a stable acyl-enzyme with a stoichiometric release of benzyl thiol. Thiol production is measured...
متن کاملCHEMICAL SYNTHESIS OF p-NITROPHENYL p-NITROPHENYL ~LAh%INARABIOSlDE*
Condensation OF tetra-U-acetyk-D-glucopyranosyl bromide withpnitrophenyl 4,6-O-benzylidene-/?-D-glucopyranoside followed by appropriate deblocking reactions gives rise to an approximately equimolar mixture of p-nitrophenyl @,ophoroside and pnitrophenyi Plaminarabioside. The two glycosides are readiIy separated and isolated in pure form by cellulose-column chromatography.
متن کاملPotassium p-nitrophenyl sulfate.
The crystal structure of the title compound, K+.C6H4NO6S-, is built up from p-nitrophenyl sulfate anions and potassium cations. Adjacent anions form dimers, which are linked together in a three-dimensional network via short C-H...O contacts. The coordination sphere of the K+ ions may be described as a distorted square antiprism. The crystal structure is further stabilized by pi-pi stacking inte...
متن کاملpart a: application of n-(p-toluenesulfonyl) imidazole (tsim) and triphenylphosphine/carbon tetrachloride in several organic transformations part b: application of 8-bromocaffeine for synthesis of some novel 8-caffeinyl derivatives
بخش اول این پایان نامه به طور عمده بر توسعه کاربردهای جدید n-(پارا-تولوئن سولفونیل) ایمیدازول (tsim) و تری فنیل فسفین/ تتراکلرید کربن در تبدیل گروههای عاملی به یکدیگر استوار است. نظر به تنوع زیاد، دردسترس بودن، سمیت کمتر و نقل و انتقال آسان تر الکل ها نسبت به آلکیل هالیدها، تبدیل مستقیم گروه هیدروکسیل به گروه های عاملی دیگر مثل آزید، نیتریل و استر یکی از مهم ترین تبدیلات در سنتزهای آلی است. با ...
15 صفحه اولThe inhibition of trypsin. IV. Reaction with diethyl p-nitrophenyl phosphate in the presence of urea.
The inhibition of proteolytic enzymes by organophosphorus compounds has been extensively studied in recent years (1, 2). Such studies have shown that the reaction of trypsin with diisopropyl fluorophosphate (3) or diethyl p-nitrophenyl phosphate (DENP) (4) is accompanied by a loss in the proteolytic, esterase, and amidase activities of this enzyme. It is not certain, however, whether these inhi...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1970
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.18.2571